Since Tails 3, you'll have to do something like gksu nautilus to launch a root nautilus. You'll get a GTK-Warning cannot open display: :1 if you have run a root file manager before in the same session
Described here:
https://docs.securedrop.org/en/latest/upgrade_to_tails_3x.html#upgrade-the-tails-drives
https://docs.securedrop.org/en/latest/backup_workstations.html

gksu nautilus from a regular terminal would work. When reproducing this bug, I found that it only happens when having run a root nautilus in the same sesion before.
Was able to reproduce on hardware. Thanks @KwadroNaut!
Seems running gksu nautilus from the regular terminal application would be a more sustainable alternative in the long run.
Currently WIP as update_tails_docs_gksudo.