Rnaseq: Test profile seems to download wrong FASTQ files

Created on 4 Jan 2021  路  2Comments  路  Source: nf-core/rnaseq

Checking the results of my test profile run against the published results, it seems that I have a different set of fastqs. My files are labeled with the convention WT_R* and RAP_*, and alignment is consistently very poor (6-8%). I would guess this is probably a genome mismatch. I previously used revision 1.4.2 fairly extensively, so it's possible these are cached somewhere, but I have removed everything from the ~/.nextflow/assets directory. Is there a way to purge these files? I just want to be sure all is running correctly.

Alignment results:

-[nf-core/rnaseq] 5/5 samples passed STAR 5% mapped threshold:
7.07%: RAP1_IAA_30M_R1
6.76%: WT_R2
6.62%: RAP1_UNINDUCED_R1
7.87%: WT_R1
6.38%: RAP1_UNINDUCED_R2

question

All 2 comments

Hi @RoganGrant ! Happy new year! Hope you and loved ones are well.

This is all expected because I updated the test dataset after 1.4.3. However, as you have observed the alignment rates are quite poor which can lead to confusion. See https://github.com/nf-core/rnaseq/issues/544

Also, the published results on the website are from the full test dataset and not from the minimal test dataset which is why the names are different as you can see in this samplesheet https://github.com/nf-core/test-datasets/blob/rnaseq/samplesheet/samplesheet_full.csv

The most important thing is that -profile test passes.

Just wanted to make sure. Thank you! And happy new year to you as well!

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