Seurat: Error in FindClusters.Seurat

Created on 22 Nov 2018  Â·  2Comments  Â·  Source: satijalab/seurat

After upgrading to version 3 I get an error when trying to find clusters
Error in FindClusters.Seurat
Provided graph.name not present in Seurat object.

Could anybody help me with this?
Thanks

Most helpful comment

see #939

In 3.0, we separate clustering into two steps. First, we construct a
neighbor graph between single cells, and we next FindClusters (credit to
scanpy for separating these two steps).

To do the first step, we run the FindNeighbors function - as documented on
our 'cheat sheet' for Seurat v3
https://satijalab.org/seurat/essential_commands.html
https://urldefense.proofpoint.com/v2/url?u=https-3A__satijalab.org_seurat_essential-5Fcommands.html&d=DwMFaQ&c=slrrB7dE8n7gBJbeO0g-IQ&r=e2kPyDtZAzQoyPfLTjZ5kw&m=-GgkxVz_gdz_QIIqTHO5p3pB4lLhTciaZib98W9dGtk&s=qBpWahyC4FgjIoGDgY5aL4_KfuPj-_TCMU-3EKYr_oM&e=

On Thu, Nov 22, 2018 at 10:16 AM rmenafra notifications@github.com wrote:

After upgrading to version 3 I get an error when trying to find clusters
Error in FindClusters.Seurat
Provided graph.name not present in Seurat object.

Could anybody help me with this?
Thanks

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All 2 comments

see #939

In 3.0, we separate clustering into two steps. First, we construct a
neighbor graph between single cells, and we next FindClusters (credit to
scanpy for separating these two steps).

To do the first step, we run the FindNeighbors function - as documented on
our 'cheat sheet' for Seurat v3
https://satijalab.org/seurat/essential_commands.html
https://urldefense.proofpoint.com/v2/url?u=https-3A__satijalab.org_seurat_essential-5Fcommands.html&d=DwMFaQ&c=slrrB7dE8n7gBJbeO0g-IQ&r=e2kPyDtZAzQoyPfLTjZ5kw&m=-GgkxVz_gdz_QIIqTHO5p3pB4lLhTciaZib98W9dGtk&s=qBpWahyC4FgjIoGDgY5aL4_KfuPj-_TCMU-3EKYr_oM&e=

On Thu, Nov 22, 2018 at 10:16 AM rmenafra notifications@github.com wrote:

After upgrading to version 3 I get an error when trying to find clusters
Error in FindClusters.Seurat
Provided graph.name not present in Seurat object.

Could anybody help me with this?
Thanks

—
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Reply to this email directly, view it on GitHub
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or mute the thread
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.

Sorry I missed that issue, thanks a lot!

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