Seurat: Merging more than two seurat objects

[EDYAC]

Is there a way to merge 4 seurat objects?
MergeSeurat is for two objects. I know that there is also AddSamples but this add a sample without creating a Seurat Object, my point is that I have 4 dataset, I want to create a Seurat object for each so I can filter the cells for each object then merge them all together. is there a way to do that?
Thank you all

[mschilli87]

@EDYAC: see https://github.com/satijalab/seurat/issues/207, https://github.com/satijalab/seurat/issues/239 & https://github.com/satijalab/seurat/issues/601 :wink:

[Dragonmasterx87]

@EDYAC I have some code that can help you merge>2 objects 🐉 You can find it here!

[Simon6089]

I think the Reduce function can work well, for example:
{r} Reduce(function(x,y) merge(x,y,add.cell.ids = c([email protected],[email protected])) , Seurat.list)
just input a list of seurat objects, and it seems that MergeSeurat function is replaced by merge.

[timoast]

@Simon6089 there's no need to do this, y can be a list of Seurat objects (see the docs for merge)