Hi,
I want to know whether one nano-sequence read (e.g. 10kb ) can be mapped to the reference sequences and is reported by minimap2, where the first 5kb is perfectly matched the reference sequence but the last 5kb is not matched.
There is no risk of a nanopore sequence aligning perfectly along 5000bp in the current universe :-)
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There is no risk of a nanopore sequence aligning perfectly along 5000bp in the current universe :-)