I know this can sometimes be dangerous ground to go to but I have a fairly straight forward question:
minimap2 flags something as a supplementary alignment, are you saying it's chimeric or that it maps to two places on the reference? Or possibly both?Supplementary (SAM 0x800) = chimeric
Secondary (SAM 0x100) = multiple mappings
Most helpful comment
Supplementary (SAM 0x800) = chimeric
Secondary (SAM 0x100) = multiple mappings